Analysis of Human Platelet Glycoproteins lib-Illa and Glanzmann’s Thrombasthenia in Whole Blood by Flow Cytometry
نویسندگان
چکیده
Antibodies that bind to human platelet membrane glycoproteins llb and lIla were used to develop methods for analyzing platelet membrane components by flow cytometry. Platelets were tentatively identified by their lowintensity light scatter profiles in whole blood or plateletrich plasma preparations. Identification of this cell population as platelets was verified by using plateletspecific antibodies and fluorescein-conjugated antiimmunoglobulin. Two-parameter analysis of light scatter versus fluorescence intensity identified >98% of the cells in the “platelet” light scatter profile as platelets due to their acquired fluorescence. Both platelet-rich plasma and whole blood were used to study platelet membrane glycoproteins IIb and lIla on a single cell basis in an unwashed system.
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